Iron oxide nanoparticles induce ferroptosis under mild oxidative stress in vitro

Aramouni, K. et al. Biochemical and cellular basis of oxidative stress: Implications for disease onset. J. Cell. Physiol. 238, 1951–1963 (2023).

Article 
CAS 
PubMed 

Google Scholar 

Martemucci, G. et al. Free radical properties, source and targets, antioxidant consumption and health. Oxygen 2, 48–78 (2022).

Article 
CAS 

Google Scholar 

Yan, L. J. Positive oxidative stress in aging and aging-related disease tolerance. Redox Biol. 2, 165–169 (2014).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Qiao, R. et al. Magnetic iron oxide nanoparticles for brain imaging and drug delivery. Adv. Drug Deliv. Rev. 197, 114822 (2023).

Article 
CAS 
PubMed 

Google Scholar 

Zhao, Z. et al. Recent advances in engineering iron oxide nanoparticles for effective magnetic resonance imaging. Bioact. Mater. 12, 214–245 (2022).

CAS 
PubMed 

Google Scholar 

Abdel Maksoud, M. I. A. et al. Engineered magnetic oxides nanoparticles as efficient sorbents for wastewater remediation: a review. Environ. Chem. Lett. 20, 519–562 (2022).

Article 
CAS 

Google Scholar 

Pucci, C., Degl’Innocenti, A., Belenli Gümüş, M. & Ciofani, G. Superparamagnetic iron oxide nanoparticles for magnetic hyperthermia: recent advancements, molecular effects, and future directions in the omics era. Biomater. Sci. 10, 2103–2121 (2022).

Article 
CAS 
PubMed 

Google Scholar 

Liu, G., Gao, J., Ai, H. & Chen, X. Applications and potential toxicity of magnetic iron oxide nanoparticles. Small 9, 1533–1545 (2013).

Article 
CAS 
PubMed 

Google Scholar 

Malhotra, N. et al. Potential toxicity of iron oxide magnetic nanoparticles: A review. Molecules 25, 3159 (2020).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Sengul, A. B. & Asmatulu, E. Toxicity of metal and metal oxide nanoparticles: a review. Environ. Chem. Lett. 18, 1659–1683 (2020).

Article 
CAS 

Google Scholar 

Nowak-Jary, J. & Machnicka, B. Pharmacokinetics of magnetic iron oxide nanoparticles for medical applications. J. Nanobiotechnol. 20, 305 (2022).

Article 
CAS 

Google Scholar 

Vakili-Ghartavol, R. et al. Toxicity assessment of superparamagnetic iron oxide nanoparticles in different tissues. Artif. Cell Nanomed. B 48, 443–451 (2020).

Article 
CAS 

Google Scholar 

Chrishtop, V. V., Mironov, V. A., Prilepskii, A. Y., Nikonorova, V. G. & Vinogradov, V. V. Organ-specific toxicity of magnetic iron oxide-based nanoparticles. Nanotoxicology 15, 167–204 (2021).

Article 
CAS 
PubMed 

Google Scholar 

Dixon, Scott J. et al. Ferroptosis: An iron-dependent form of nonapoptotic cell death. Cell 149, 1060–1072 (2012).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Dixon, S. J. & Olzmann, J. A. The cell biology of ferroptosis. Nat. Rev. Mol. Cell. Biol. 25, 424–442 (2024).

Article 
CAS 
PubMed 

Google Scholar 

Liu, Y. & Wang, J. Multivalent metal catalysts in Fenton/Fenton-like oxidation system: A critical review. Chem. Eng. J. 466, 143147 (2023).

Article 
CAS 

Google Scholar 

Kurz, T., Terman, A. & Brunk, U. T. Autophagy, ageing and apoptosis: The role of oxidative stress and lysosomal iron. Arch. Biochem. Biophy. 462, 220–230 (2007).

Article 
CAS 

Google Scholar 

Repetto, M. G., Ferrarotti, N. F. & Boveris, A. The involvement of transition metal ions on iron-dependent lipid peroxidation. Arch. Toxicol. 84, 255–262 (2010).

Article 
CAS 
PubMed 

Google Scholar 

Huang, C.-C. et al. Cellular organelle-dependent cytotoxicity of iron oxide nanoparticles and its implications for cancer diagnosis and treatment: A mechanistic investigation. Chem. Mater. 28, 9017–9025 (2016).

Article 
CAS 

Google Scholar 

Iavicoli, I., Fontana, L. & Nordberg, G. The effects of nanoparticles on the renal system. Crit. Rev. Toxicol. 46, 490–560 (2016).

Article 
CAS 
PubMed 

Google Scholar 

Wen, X. et al. Tumor microenvironment cascade activated biodegradable nano-enzymes for glutathione-depletion and ultrasound-enhanced chemodynamic therapy. Small https://doi.org/10.1002/smll.202405457 (2024).

Article 
PubMed 

Google Scholar 

Gu, J. et al. Cerium–luteolin nanocomplexes in managing inflammation-related diseases by antioxidant and immunoregulation. ACS Nano 18, 6229–6242 (2024).

Article 
CAS 
PubMed 

Google Scholar 

Srivastava, A., Tomar, B., Sharma, D. & Rath, S. K. Mitochondrial dysfunction and oxidative stress: Role in chronic kidney disease. Life Sci. 319, 121432 (2023).

Article 
CAS 
PubMed 

Google Scholar 

Schulz, M.-C. et al. Acidosis activates the Nrf2 pathway in renal proximal tubule-derived cells through a crosstalk with renal fibroblasts. Antioxidants 12, 412 (2023).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Guo, M. et al. High glucose-induced kidney injury via activation of necroptosis in diabetic kidney disease. Oxid. Med. Cell. Longev. 2023, 2713864 (2023).

Article 
PubMed 
PubMed Central 

Google Scholar 

Luo, C. et al. Superparamagnetic iron oxide nanoparticles exacerbate the risks of reactive oxygen species-mediated external stresses. Arch. Toxicol. 89, 357–369 (2015).

Article 
CAS 
PubMed 

Google Scholar 

Ghasemi, M., Liang, S., Luu, Q. M. & Kempson, I. Cell Viability Assays: Methods and Protocols (Springer, 2023).

Google Scholar 

Wojtala, A. et al. Methods in Enzymology (Academic Press, 2014).

Google Scholar 

Li, Z. et al. Pterostilbene upregulates MICA/B via the PI3K/AKT signaling pathway to enhance the capability of natural killer cells to kill cervical cancer cells. Exp. Cell. Res. 435, 113933 (2024).

Article 
CAS 
PubMed 

Google Scholar 

Sivandzade, F., Bhalerao, A. & Cucullo, L. Analysis of the mitochondrial membrane potential using the cationic JC-1 dye as a sensitive fluorescent probe. Bio Protocol. 9, e3128 (2019).

Article 
PubMed 
PubMed Central 

Google Scholar 

Jesus, A. D. et al. Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay. J. Vis. Exp. https://doi.org/10.3791/61122-v (2020).

Article 

Google Scholar 

Thomé, M. P. et al. Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy. J. Cell Sci. 129, 4622–4632 (2016).

Article 
PubMed 

Google Scholar 

Liao, M. et al. METTL3-mediated m6A modification of NORAD inhibits the ferroptosis of vascular smooth muscle cells to attenuate the aortic dissection progression in an YTHDF2-dependent manner. Mol. Cell. Biochem. 479, 3471–3487 (2024).

Article 
CAS 
PubMed 

Google Scholar 

Ma, P.-W. et al. Treatment with the ferroptosis inhibitor ferrostatin-1 attenuates noise-induced hearing loss by suppressing ferroptosis and apoptosis. Oxid. Med. Cell Longev. 2022, 3373828 (2022).

Article 
PubMed 
PubMed Central 

Google Scholar 

Frtús, A. et al. Analyzing the mechanisms of iron oxide nanoparticles interactions with cells: A road from failure to success in clinical applications. J. Control Release 328, 59–77 (2020).

Article 
PubMed 

Google Scholar 

Etemadi, H., Buchanan, J. K., Kandile, N. G. & Plieger, P. G. Iron oxide nanoparticles: Physicochemical characteristics and historical developments to commercialization for potential technological applications. ACS Biomater. Sci. Eng. 7, 5432–5450 (2021).

Article 
CAS 
PubMed 

Google Scholar 

Hajam, Y. A. et al. Oxidative stress in human pathology and aging: Molecular mechanisms and perspectives. Cells 11, 552 (2022).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Stockwell, B. R. Ferroptosis turns 10: Emerging mechanisms, physiological functions, and therapeutic applications. Cell 185, 2401–2421 (2022).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Scarpellini, C. et al. Beyond ferrostatin-1: a comprehensive review of ferroptosis inhibitors. Trends Pharmacol. Sci. 44, 902–916 (2023).

Article 
CAS 
PubMed 

Google Scholar 

Donaldson, K., Stone, V., Tran, C. L., Kreyling, W. & Borm, P. J. A. Nanotoxicology. Occup. Environ. Med. 61, 727 (2004).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Huang, X., Li, X. & Tay, A. Advances in techniques to characterize cell-nanomaterial interactions (CNI). Nano Today 55, 102149 (2024).

Article 
CAS 

Google Scholar